Development of online-measurement techniques for miniaturized Bioreactors using multiwavelength fluorescence spectroscopy

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Christoph Berg

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+49 241 80 23566

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Due to the complexity of biological systems, determining optimal growth conditions is a major challenge for bioprocess engineering. For minimizing the time needed for development, it is of big interest to maximize the experimental throughput without losing relevant process information. Consequently, during the last decades, effort were taken to develop parallelized small-scale cultivation systems. Based on the Process Analytical Technology (PAT) Initiative of 2002 central aspects are automation, miniaturization and potential for online monitoring.
With the BioLector Technology, the chair of Biochemical Engineering has developed a measurement technique, which provides non-invasive in-situ monitoring of microbial cultivation processes in milliliter scale. The implemented optical setup allows a fast data recording for both scattered and fluorescent light in a continuous wavelength window of the visible light. Here, especially the fluorescing activity of cellular compounds give information on the composition of the culture broth at any time of the production process. However, with the developed measuring technique, large experimental datasets are gathered, which hardly can be unraveled and visualized by conventional analysis methods. For maximizing the degree of data exploitation, applying methods of chemometry such as multivariate data analysis and regression are of high interest thus minimizing the need of offline measurements.
At the chair of Biochemical Engineering research concentrates on the development of the BioLector technology using 2D Fluorescence spectroscopy and the potentials of multivariate data analysis for processing of the experimental datasets.